diff --git a/test/e2e/internal/precheck/affinity_toleration.go b/test/e2e/internal/precheck/affinity_toleration.go
new file mode 100644
index 0000000000..846a1f45ba
--- /dev/null
+++ b/test/e2e/internal/precheck/affinity_toleration.go
@@ -0,0 +1,107 @@
+/*
+Copyright 2026 Flant JSC
+
+Licensed under the Apache License, Version 2.0 (the "License");
+you may not use this file except in compliance with the License.
+You may obtain a copy of the License at
+
+    http://www.apache.org/licenses/LICENSE-2.0
+
+Unless required by applicable law or agreed to in writing, software
+distributed under the License is distributed on an "AS IS" BASIS,
+WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+See the License for the specific language governing permissions and
+limitations under the License.
+*/
+
+package precheck
+
+import (
+	"context"
+	"fmt"
+
+	"github.com/deckhouse/virtualization/test/e2e/internal/framework"
+	. "github.com/onsi/ginkgo/v2"
+	corev1 "k8s.io/api/core/v1"
+	crclient "sigs.k8s.io/controller-runtime/pkg/client"
+)
+
+const (
+	affinityTolerationPrecheckEnvName   = "AFFINITY_TOLERATION_PRECHECK"
+	affinityTolerationNodeGroupLabelKey = "node.deckhouse.io/group"
+	affinityTolerationKVMLabelKey       = "virtualization.deckhouse.io/kvm-enabled"
+
+	minReadyKVMNodes       = 2
+	minReadyKVMMasterNodes = 1
+	minReadyKVMWorkerNodes = 2
+)
+
+// affinityTolerationPrecheck implements Precheck interface for VM affinity/toleration test cluster requirements.
+type affinityTolerationPrecheck struct{}
+
+func (a *affinityTolerationPrecheck) Label() string {
+	return PrecheckAffinityToleration
+}
+
+func (a *affinityTolerationPrecheck) Run(ctx context.Context, f *framework.Framework) error {
+	if !isCheckEnabled(affinityTolerationPrecheckEnvName) {
+		_, _ = GinkgoWriter.Write([]byte("Affinity/toleration precheck is disabled.\n"))
+		return nil
+	}
+
+	kvmNodes, err := listReadyNodesByLabels(ctx, f, map[string]string{affinityTolerationKVMLabelKey: "true"})
+	if err != nil {
+		return fmt.Errorf("%s=no to disable this precheck: failed to list ready KVM-enabled nodes: %w", affinityTolerationPrecheckEnvName, err)
+	}
+	if len(kvmNodes) < minReadyKVMNodes {
+		return fmt.Errorf("%s=no to disable this precheck: at least %d ready KVM-enabled nodes are required, got %d", affinityTolerationPrecheckEnvName, minReadyKVMNodes, len(kvmNodes))
+	}
+
+	masterNodes, err := listReadyNodesByLabels(ctx, f, map[string]string{
+		affinityTolerationKVMLabelKey:       "true",
+		affinityTolerationNodeGroupLabelKey: "master",
+	})
+	if err != nil {
+		return fmt.Errorf("%s=no to disable this precheck: failed to list ready KVM-enabled master nodes: %w", affinityTolerationPrecheckEnvName, err)
+	}
+	if len(masterNodes) < minReadyKVMMasterNodes {
+		return fmt.Errorf("%s=no to disable this precheck: at least %d ready KVM-enabled master node is required, got %d", affinityTolerationPrecheckEnvName, minReadyKVMMasterNodes, len(masterNodes))
+	}
+
+	workerNodes, err := listReadyNodesByLabels(ctx, f, map[string]string{
+		affinityTolerationKVMLabelKey:       "true",
+		affinityTolerationNodeGroupLabelKey: "worker",
+	})
+	if err != nil {
+		return fmt.Errorf("%s=no to disable this precheck: failed to list ready KVM-enabled worker nodes: %w", affinityTolerationPrecheckEnvName, err)
+	}
+	if len(workerNodes) < minReadyKVMWorkerNodes {
+		return fmt.Errorf("%s=no to disable this precheck: at least %d ready KVM-enabled worker nodes are required, got %d", affinityTolerationPrecheckEnvName, minReadyKVMWorkerNodes, len(workerNodes))
+	}
+
+	return nil
+}
+
+func listReadyNodesByLabels(ctx context.Context, f *framework.Framework, labels map[string]string) ([]corev1.Node, error) {
+	nodes := &corev1.NodeList{}
+	err := f.GenericClient().List(ctx, nodes, crclient.MatchingLabels(labels))
+	if err != nil {
+		return nil, err
+	}
+
+	readyNodes := make([]corev1.Node, 0, len(nodes.Items))
+	for _, node := range nodes.Items {
+		for _, condition := range node.Status.Conditions {
+			if condition.Type == corev1.NodeReady && condition.Status == corev1.ConditionTrue {
+				readyNodes = append(readyNodes, node)
+				break
+			}
+		}
+	}
+
+	return readyNodes, nil
+}
+
+func init() {
+	RegisterPrecheck(&affinityTolerationPrecheck{}, false)
+}
diff --git a/test/e2e/internal/precheck/labels.go b/test/e2e/internal/precheck/labels.go
index b18e131722..5d5bf6dbf4 100644
--- a/test/e2e/internal/precheck/labels.go
+++ b/test/e2e/internal/precheck/labels.go
@@ -45,6 +45,9 @@ const (
 	// PrecheckUSB - test requires USB device with dummy_hcd to be configured.
 	PrecheckUSB = "usb-precheck"
 
+	// PrecheckAffinityToleration - test requires enough ready KVM-enabled master/worker nodes.
+	PrecheckAffinityToleration = "affinity-toleration-precheck"
+
 	// PrecheckPostCleanup - test requires postcleanup to be configured.
 	PrecheckPostCleanup = "postcleanup-precheck"
 
@@ -68,6 +71,7 @@ func KnownPrecheckLabels() []string {
 		PrecheckSnapshot,
 		PrecheckVirtualization,
 		PrecheckUSB,
+		PrecheckAffinityToleration,
 		PrecheckPostCleanup,
 		PrecheckPrecreatedCVI,
 		NoPrecheck,
diff --git a/test/e2e/vm/affinity_toleration.go b/test/e2e/vm/affinity_toleration.go
index fa78e64972..5f21d482a6 100644
--- a/test/e2e/vm/affinity_toleration.go
+++ b/test/e2e/vm/affinity_toleration.go
@@ -55,7 +55,7 @@ const (
 	migrationTargetMustDiffer
 )
 
-var _ = Describe("VirtualMachineAffinityAndToleration", Ordered, Label(precheck.NoPrecheck), func() {
+var _ = Describe("VirtualMachineAffinityAndToleration", Ordered, Label(precheck.PrecheckAffinityToleration), func() {
 	var (
 		f   *framework.Framework
 		ctx context.Context